منابع مشابه
Clonal assay of mouse mast cell colonies in methylcellulose culture.
When mouse marrow and spleen cells were cultured for over 12 days in methylcellulose containing media conditioned by pokeweed-mitogen-stimulated spleen cells, colonies containing mast cells and blast cells were observed. The characteristic morphology of the colonies and the time course of their development allowed in situ identification of the mast cell colonies. Identification of the mast cell...
متن کاملMethylcellulose media for plaque assay of murine leukemia virus.
When ecotropic murine leukemia virus was assayed by a methylcellulose-XC cell procedure, plaque titers showed less test-to-test variation, more uniform dose-response curves, and larger plaque sizes, as compared with results of the conventional liquid overlay-XC cell test system. This assay therefore seems to be reliable and useful for the titration of ecotropic murine leukemia virus.
متن کاملThe Dlk1-Gtl2 Locus Preserves LT-HSC Function by Inhibiting the PI3K-mTOR Pathway to Restrict Mitochondrial Metabolism.
The mammalian imprinted Dlk1-Gtl2 locus produces multiple non-coding RNAs (ncRNAs) from the maternally inherited allele, including the largest miRNA cluster in the mammalian genome. This locus has characterized functions in some types of stem cell, but its role in hematopoietic stem cells (HSCs) is unknown. Here, we show that the Dlk1-Gtl2 locus plays a critical role in preserving long-term rep...
متن کاملLT Codes
We introduce LT codes, the first rateless erasure codes that are very efficient as the data length grows.
متن کاملHSC Niche Biology and HSC Expansion Ex Vivo.
Hematopoietic stem cell (HSC) transplantation can restore a new functional hematopoietic system in recipients in cases where the system of the recipient is not functional or for example is leukemic. However, the number of available donor HSCs is often too low for successful transplantation. Expansion of HSCs and thus HSC self-renewal ex vivo would greatly improve transplantation therapy in the ...
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ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2014
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.1067